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gfp trap agarose  (Proteintech)


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    Structured Review

    Proteintech gfp trap agarose
    Gfp Trap Agarose, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+trap+agarose/GFP+tag+Antibody/pm41914604-278-6-11
    Average 96 stars, based on 1652 article reviews
    gfp trap agarose - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Protein-Protein interactions:

    Article Title: VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons
    Article Snippet: Cells were briefly washed, scraped, and centrifugated in ice-cold 1xPBS and subsequently lysed in AP-MS buffer (10 mM Tris/Cl, pH 7.5, 150 mM NaCl, 10% glycerol, 0.5 mM EDTA, 0.5% Nonidet P40) with protease inhibitor cocktail (cOmplete; Millipore) by sonification and incubated on ice for 1 h. Cell lysates were centrifugated at 20,000 × g for 10 min at 4°C. .. Magnetic c-Myc (88843; Thermo Fisher Scientific) or GFP-Trap Agarose (gta-20; ChromoTek) beads were blocked with 5% BSA in AP-MS buffer (2 h, 4°C) prior to IP. .. Magnetic c-Myc (88843; Thermo Fisher Scientific) or GFP-Trap Agarose (gta-20; ChromoTek) beads were blocked with 5% BSA in AP-MS buffer (2 h, 4°C) prior to IP.

    Lysis:

    Article Title: Modulation of Salmonella virulence by a novel SPI-2 injectisome effector that interacts with the dystrophin-associated protein complex
    Article Snippet: .. GFP-trap agarose (Chromotek) or anti-Flag M2 affinity gel (Sigma) were used to immuno-precipitate GFP-tagged protein or Flag-tagged protein by using buffer B (5% glycerol, 0.5% Triton X-100, 1 mM phenylmethylsulfonyl fluoride [PMSF], and PBS) as lysis buffer and buffer C (5% glycerol, 0.1% Triton X-100, 1 mM PMSF, and PBS) as washing buffer. ..

    Incubation:

    Article Title: Histone H3K27 demethylase SlJMJ3 modulates fruit ripening in tomato.
    Article Snippet: The histone lysine (K) demethylase 4 (KDM4/JHDM3) subfamily of jumonji domain–containing demethylases (JMJs) has been implicated in various aspects of plant development.. However, their involvement in regulating the ripening of fleshy fruits remains unclear.. In this study, we identified SlJMJ3, a member of the KDM4/JHDM3 family, as an H3K27me3 demethylase in tomato (Solanum lycopersicum) that plays an important role in fruit ripening regulation.

    Immunoprecipitation:

    Article Title: Histone H3K27 demethylase SlJMJ3 modulates fruit ripening in tomato.
    Article Snippet: The histone lysine (K) demethylase 4 (KDM4/JHDM3) subfamily of jumonji domain–containing demethylases (JMJs) has been implicated in various aspects of plant development.. However, their involvement in regulating the ripening of fleshy fruits remains unclear.. In this study, we identified SlJMJ3, a member of the KDM4/JHDM3 family, as an H3K27me3 demethylase in tomato (Solanum lycopersicum) that plays an important role in fruit ripening regulation.

    Chromatin Immunoprecipitation:

    Article Title: SbWRKY50 Promotes Drought Tolerance of Sorghum by Regulating Lateral Root Formation.
    Article Snippet: .. GFP‐ Trap Agarose (gta‐20, ChromoTek) were used for ChIP. .. DNA fragments were purified using the PCR Purification Kit (#K0702, Thermo).



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